Tuna is one of the most widely consumed fish on the European market, being available in various IBS RELIEF consumable options.Among them, Thunnus albacares, also called yellowfin tuna, is a delicacy and is consumed by millions of people around the world.Due to its comparatively high cost and demand, it is more vulnerable to fraud, where low-cost tuna or other fish varieties might be replaced for economic gain.In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and validated for targeting the mitochondrial cytochrome b gene for fast and direct detection of Thunnus albacares, which is a valuable tuna species.The analytical specificity was confirmed using 18 target samples (Thunnus albacares) and 18 samples of non-target fish species.
The analytical sensitivity of the LAMP assay was 540 fg DNA Videos per reaction.In addition, a simple and direct swab method without time-consuming nucleic acid extraction procedures and the necessity for cost-intensive laboratory equipment was performed that allowed LAMP detection of Thunnus albacares samples within 13 minutes.Due to its high specificity and sensitivity, the LAMP assay can be used as a rapid and on-site screening method for identifying Thunnus albacares, potentially providing a valuable monitoring tool for food authenticity control by the authorities.